Cocaine

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Definition Essay Cocaine

Cocaine is a bitter crystalline alkaloid obtained from coca leaves and synthesized from ecognine. If taken internally it has first a stimulating and then a narcotic effect. In large doses cocaine produces intoxication and acts as a local anesthetic. The history of how powder cocaine came to be one of the worlds biggest drug problems is shown in the movie Blow, directed by Ted Demme. George Jung is a character that moves to California to find a way to make a living other than being a construction worker like his father. He is introduced to selling marijuana and becomes involved in very heavy dealing. When he is busted and sent to prison for the first time he learns about the smuggling of cocaine from Columbia's medellin cartel. In the business of his smuggling he meets Mirtha, a girl who he later marries and has a daughter with. His daughter, Kristina Sunshine, brings a new perspective in George's mind. She is the greatest love if his life and he wants to more than just a criminal in her eyes. Before he can change anything he falls into the hands of the feds once again. Cocaine becomes such a big part of George's life it starts to effect him, his daughter, and his parents, in all the wrong ways.

The word cocaine is a noun which has its roots from "coca" and the use of -ine which comes from -inus or -ina for the ending of a noun. It comes from France in 1856. The root "coca" comes form Quechua or "cuca". Coca is the name of the plant from which the drug cocaine is derived.

Cocaine effects the life of George Jung in many ways. He is sucked into the life of drug addiction, and the dangerous ways of being the worlds premiere importer of cocaine. Money becomes the center of everything. He was bringing in 5 billion dollars a year and eventually had to buy a new house just to hold all of the wall-to-wall cash he had at his hands. He was dealing with some of the most dangerous men in the world everyday and was always at the risk of being caught. In the midst of everything his wife was addicted, he was addicted, and they were raising a child in a world of crime, violence, addiction, and things children should never be exposed to. George's life could only go downhill and eventually is caught by the feds and taken from the one thing that mattered the most in his life, his daughter Kristina.

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George's daughter Kristina Sunshine is brought into the world seeing her father as a criminal, her mother and an addict, and never knowing that it was wrong. George has such a great love for Kristina and would never put her in danger or intentionally hurt her in any way. She is in no real danger when she is with him but only ends up being hurt emotionally by the events of her fathers life. When he sent to prison she is abruptly taken away, but soon comes home to her mother. When her father gets out of prison he is able to her every once in a while for short periods of time. Her parents are no longer together and she does not even get to know her father any longer before he is caught for the last time. This time only breaking promises and shattering their relationship once again. Cocaine ruins her life through her parents. No child should have to see the things she saw and go through the pain she went through.

George's parents are faced with a big decision about what to think of their son. They wonder where he gets all of his luxuries and money but you can tell they know deep down what is really going on. His dad chooses to love him regardless of what he does. He does not support the role of cocaine in George's life but he does not love his son any less. His mom on the other hand becomes completely embarrassed ashamed of the son she thought she has raised so well. Knowing that any one she knows might think less of her she completely disowns her son. Not only does she disown him she intentionally tires to hurt him. He comes to visit his parents who he hasn't seen in a long time. His mother is very cold and quiet with him. While he is talking to his dad she calls the police and turns her own child in. As much as she disapproves of what George does it is wrong to want your child to suffer in prison. Cocaine has completely changed her thoughts of her son and shamed the rest of his family for the rest of their lives.

The movie showed every aspect of how your life could possibly be ruined by the drug cocaine. It showed how brutally it can hurt someone's life even if they are not in direct contact with the drug. Cocaine has ruined George's life. He is in prison, suffering for what he did in his lifetime. Kristina will always be emotionally scarred from the events of her lifetime. And lastly, his parents will always have pity and shame for their son and what the cocaine did to his life.

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Analysis of Sid from Good as Gold

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Character Analysis of Sid

Sid was the firstborn of the Gold Family. It is described that most of his physical characteristics are attributed from the genes of his father. Heavy Set, with gray parting hair. As the story progresses, Sid takes on most of the stylish attributes of his father, as well, in the way he dresses and settles in his life with his wife, Harriet.

Sid's behavior could possibly have some anti-societal tendencies as he enters his adult life. Some of his characteristics are very egocentric with no empathy for those not in his family, and he appears incapable of feeling remorse or guilt. In the business world he is definitely a player for the good of his family. His behavior with family members is confusing. Sid is capable of being in complete charge of the situation, whatever it may be. Whether he is or not, to his family he appears to be. Throughout the storyline this is played through in his personality. In addition, he is very quick to give counsel. In his private life, there are times when it seems rather disorganized.

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Sid contains some socialization problems due to his childhood, and being raised as a Jew. Because of being called a "JewBoy" (p. 5), being spit on, and never having any true friends while a child because he was classified as a Jew. Sid became an expert at making his existence valuable in his adult years using his first-born male status as the backdrop. He did not allow his true feelings of rejection be shown until he was ready in his senior years.

Sid was at times impulsive, manipulative, reckless, and quarrelsome. He believed strongly in his long-term commitment with his wife Harriet. In his eyes, this is what is expected of him from the other family members since he again was the first-born and a male and role model for his siblings. Sid was manipulative in convince his co-workers, family and his wife that he was away on business, when he was actually sporting the good life in a completely different place. As time moved forward Sid would reconcile with age, leaving the more freethinking life behind. Sid became with age, not as open minded to taking such chances.

Sid was an excellent actor, always appearing charming, calm, and collected. Sid clearly shows this characteristic while having a discussion with his father and brother on page 6. His father reminds him that he has indeed helped him financially. During this conversation, Sid clearly cannot handle the tone of disappointment in the discussion. Sid change's the focus, trying to give advise to his father as to investing in a condo. Sid's father has taken the bait. The dialogue quickly changes to a choice in investments and the attention is no longer on Sid and what he has, or has not done.

Although Sid has not attended college, he does have a normal or above normal intelligence level and good verbal fluency. It is these qualities that sometimes place him in leadership positions within social groups and often make it hard to spot his black side.

Sid appears to be very normal, calm, and educated on the exterior, but on the interior, he is incapable of experiencing any form of emotional content. He rarely comes in contact with the law, but carries a personality that if he should Sid would be able to talk him self out of the dilemma using his verbal skills.

Although not the main character, Sid plays a very important rose in "Good As Gold" in understanding the family life. His persona is reflected as an active family member. His intentions are always for the good, and wanting to be perfect in the first-born role. He is a good listener, advisor and player throughout the entire storyline.

Gold, the main character and his brother Sid experience a sort of exchange of wits while Gold is testing Sid to his own personal regrets. On the subject of not having a college education, Sid, fires back, however, the response is so calm and appears to be thought out that Gold silently accepts that the true answer is one of disappointment.

In general, his brother does not like Sid, but like any younger sibling still seeks his guidance and advise. Many times throughout the storyline the bonding of being brothers is clear despite personality difference. Sid is very much looked up to by his Stepmother and Sisters. His Stepmother highly values his opinion. It is described on page 6 that the others were struck with wonder by Sid's eloquence and pantheistic wisdom.

There is an important relation between culture and personality. Sid sees problems clearly and delegate easily, works hard and plays with zest. He willingly provides service and expects the same from others. His sense of right and wrong wrestles with an overwhelming rescuing, mothering drive. He perceives that the world is a dangerous place, not to be trusted outside of the family. He will discretely protect his family from outside the home and from inside the family home still needs the bond of his family to consider himself a success.

Because Sid carried so many characteristics that we can all relate to, he was an easy person to like and it was enjoyable and heartwarming to watch the internal relations from within his family.

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The black robe

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The Black Robe deals with the clash of cultures in a way that raises many questions in the viewers mind. The basic differences of each culture in terms of language, norms, beliefs and values, led to many misunderstandings and tragedies.

The basic premise of the movie, which is the foundation for both the action and ideas presented, is the Jesuits mission to convert the Native American Indians to Catholicism. The Jesuits believed that any baptized "savage" would automatically go to heaven upon death. For instance, in the movie the French mayor in New France introduced Black Robe and his aide as "Soldiers of Paradise", "they are here to show you the true way of God". In one of the other scenes in France, a Senior Priest says to his altar boy that the Jesuits "must convert the savages (just as they did in England and Germany)". "What more glorious a task than to convert the savages- to civilize them", so they can enter heaven.

The Algonquians, however, have their own set of beliefs, which the Jesuits find impossible to credit. For instance, the Jesuits believe in "Paradise" as "Happiness and peace, alone with God", whereas the Algonquin fail to believe there could be happiness without women, tobacco and hunting. Their idea of the "Afterworld" is "a forest where the dead see at night, and the souls of men hunt the souls of animals".

Given the basic fundamentals in their belief systems, it's no surprise that language should prove to be a problem and that misunderstandings occur in translations. For example, the Algonquians think that the mantle-clock is the leader of the New French village. When it says "Dong! Dong! Dong!" it is giving the mayor instructions (i.e.- it's "time for you to go").

At one point, Black Robe's young assistant asks, "Aren't the Algonquin 'true Christians'?" "They live for each other, and they forgive things we would never forgive". They share everything they have without question. Black Robe had a hard time understanding this as his European values stressed personal wealth and trading. For example, when Black Robe wouldn't give his tobacco to the Algonquians for reward, it was because he was going to trade it with the Huron. He believed that the Indians behave like children, with no thought for the future, and that "the devil prevents them from hearing the truth".

As with language and values, the differences in beliefs also cause tension between the Algonquin and Jesuits. Many times near the beginning of the film, Black Robe and his aide asks an Algonquin for his or her name, and they refuse to answer, claiming, "Names are sacred". Another belief the Algonquians have is related to their dreams. While the Jesuits look upon dreams as silly and meaningless, the Algonquians feel that dreams "are real, and must be obeyed". An example of this is near the end of the film, when Black Robe is told he must visit the Huron Mission alone, for it was like that in the Chief's dream. The most significant difference between the two groups' beliefs is processing of the dead. This difference creates a turning point near the middle of the film. An old Algonquin woman puts her dead baby in a tree, so it can be closer to its "Afterworld". Black Robe sees this and decides to bless the child, but this offends and angers the Algonquians, as they feel Black Robe is "stealing the child's spirit" spirit". It is after this event that Black Robe is deserted by the Algonquians and left to die.

After Black Robe is reunited with a few of the Algonquians, we are introduced to the Iroquois. They kill the Algonquian party without provocation. They take the survivors back to their camp, in preparation to sacrifice them to their own God, Arskui. They show no mercy for their captives. They treat the Jesuits and Algonquians with equal savagery. These incidents beg the question, "Does any one group have a right to impose their values on another?", which of course has been the premise of the movie all along. "The Jesuits believe the Indians are savages," and seek to change them. Whereas "the Algonquians believe the Jesuits are fools" but they don't wish to change them. For example, right before the Algonquian Chief dies, Black Robe tells the Chief that if he allows Black Robe to baptize him, he will go to "Paradise". The Chief answers by asking, "Why would I want to go to 'Paradise'? Are any of my people there? There are only black robes there".

As chilling as the treatment of the Algonquin party by the Iroquois is, it is nothing compared to the bleakness of the fate of the Huron. Near the end of the film, we see two Huron discussing the possibility of conversion. The Chief says, "Black Robe wants us to forget our dream. Have only one wife. Not to kill our enemies. But if we do that- we will cease to be Huron. Our enemies will sense weakness and wipe us off the face of the earth". A grim prophesy, which is proved true at the conclusion of the film. The Huron do convert to Catholicism, and fifteen years later, their enemies, the Iroquois, wipe them out.

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Animal farm

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Animal Farm; by George Orwell

Animal Farm, written by George Orwell, is a masterpiece mocking communism. Animalism, which represents communism, was a revolution that didnt work. Animalism was supposed to make life better for the animals but instead their lives got worse. By the end of the story, everything had changed. The government had become corrupt, there was a dictator, and the animals had become slaves to the pigs. Life for the animals couldnt get any worse. The main theme of this story is about the fall of communism and how power corrupts people.

At the beginning of the story, the animals revolted because of the way they were treated by Mr. Jones, the farmer. They felt that the farmers made all the profit, reaped all the rewards but didnt do any of the work. So they formed a government called Animalism. In Animalism, there are no owners, no rich, but no poor, workers got a better life, and all animals are equal. They had even established laws called the Seven Commandments, which were intended to give basic rights to animals and protect them from oppression. The goals of the government were also established. The goals said that everyone was equal, there would be more food and sleep for all, there was to be respect for all animals, and they would build a windmill to make life better for all. By the end of the book, all this no longer existed. The animals were getting less sleep, less food, and less respect. The windmill became a source of money for the leaders, not for all the animals. The seven commandments were gradually changed to suit the pigs and then there was only one Commandment left. It ran All animals are equal but some are more equal than others(1). That single commandment made the pigs more powerful. Animalism no longer existed.

At the beginning of the story, there were two leaders, Snowball and Napoleon, that were sharing power. Snowball was good with words, honest, good at arguing, was inventive, and believed in technology. He stayed in touch with the animals, and wanted to make things better for them. Napoleon, on the other hand, was bad with words, dishonest, hated arguing, and was not inventive. He wanted to be above all the animals; he didnt care about making things better. He only believed in serving himself. In order for Napoleon to be above all the animals, he had to get Snowball out of the way. Napoleon did that by getting his dogs to scare him away so Snowball would never come back to the farm. Napoleon was now in total control of the farm and the animals. Napoleon and the pigs started acting like humans - they would drink, wear clothes, sleep in beds, fight, and walk. They did everything that they had once said was wrong. Things were worse for the rest of animals than when Mr. Jones was running the farm.

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Just after the revolution, the animals worked on the farm because they wanted to. All the animals owned the farm, so all worked for it. It was their farm. After Napoleon takes over and creates a dictatorship, the animals are forced to work. Napoleon and the pigs owned the farm and the others became slaves to them. The animals volunteered or they got less food. The dogs used force; and Squealer, a pig, would lie to them to make them work.

In conclusion, the story Animal Farm was based on the history of communism. It used Napoleon to portray Joseph Stalin. The main point of the book was to show how the pigs had created a revolution where they gained power but let the power corrupt them into ways that were good for them but not for others. The theme of the book was to show how power can corrupt people and the reason for the fall of communism because of the corruption of power amongst Joseph Stalin.

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John Winthrops Confusion

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John Winthrop, in the 1700 thought that his religion was getting out of hand under the king and he wanted to establish a new community which would be Bible commonwealth. I intend to prove that the European diseases, that was carried over to the New World was not only a big help for them, but at the same time very new and lethal for the Native American.

It was an advantage or rather miraculous as John Winthrop puts it, seeing as how a mass majority of the Natives (savages) were taken out by smallpox, just in time for him and his Puritan crew (believers) to move in. He believed that because his cause was just, (as in creating a new colony with a holier people) he thought that God was helping him take out these unchristian savages so that he can begin setting up his new puritan colony. Not only that, with God on his side all the better for him to feel superior and to feel confident about his decision of his new colony. Going more in-depth John Winthrop was a well-educated man, his father was wealthy enough to leave him a manor, and was able to attend a good university to learn more about Christianity because the more you know the better your status becomes in the society. At the age of fifteen he got married. He kept a diary and there he would have a list of the good deeds he would do and of all the bad deeds in which he would constantly prosecute himself. He would try to think of ways to make his good list longer. It was said that only the highly educated and wealthy were those who kept diaries. He believed that under the king life was getting too liberal which forced him into the idea of making a new colony where the Bible would be law, it would then be a homogeneous society. When he started planning the colony, he advertised the standards and attracted many people to his planned out sect. Puritans believed in predestination, and that good works would win them Gods favor. The more good work you do, the more you had a chance to be considered as one of the "selected" by a minister of the colony. Religion was always an issue, if you were an outsider you would be considered uncivilized or unchristian, but if for once these Puritan and Papists and Catholics would have just look pass their superiority they would have seen that God had been through the villages of these Natives of the New World.

When the Spanish Colony decided that they would take over these Arawaks, Christopher Columbus's main concern was gold, and how easy it would be to convert these savages into Christians. Queen Isabella liked the idea of slaves and gold, so Columbus returns in 14, and the result, depopulation, suicide, disruption of the life style, malnutrition and epidemics and eventual extermination of that race. The Spaniards wiped out a whole ethnic culture. In the English's eyes the Spanish papist were nothing more than tyrannous, their contact with the Natives was disastrous. They were the beneficiaries. There wasn't one religious community that didn't feel themselves better than the other. How could change come about with feelings such as these?

The Europeans on the other hand brought diseases to the New World because of their lifestyle. In the 1500's they were said to be very nasty; they wouldn't take baths for months, where as the Native of the New World took baths regularly. And because of their very disorderly lives, it was very easy for epidemics to break out, I would imply that where there's filth there would be sickness. Smallpox was one of the diseases they would bring and actually use as a weapon to kill the Natives of the New World. Order Custom Essay on John Winthrops Confusion

The Natives were used to simple lives, they had their religion, and it didn't have a specific name; but they had their beliefs. Back in the 100 the first Natives believed that there wasn't a secular world and they believed that violating the balance of the world the spirits of the world would strike back. They weren't very knowledgeable. And breakouts of epidemics among these Natives aren't mentioned.

In balancing out what was quoted by John Winthrop, seemed to me as a win, loose, situation with the Natives. They got wipeout catastrophically by the smallpox disease, and their land was invaded. And the small amount of Natives that stayed had no choice but to submit to their Puritan way of living. There was really never any common ground with these European invaders and the Natives. The only change that came to be is that the different colonies that interacted with each other became to hate one another more and more. I would say that because of the diseases among the European it was a bonus silent weapon they had to use against the Natives.

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Abortion:Yes or No

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Abortion

I have finally decided that I should tackle an issue of grave importance in the world today. That issue is abortion. You have heard the arguments from all sides I am sure. It is a personal choice. It is murder. It is something the government should have no place in. It is something the government should be involved in. It is something I can tolerate but I do not want my tax dollars going to fund it. What stand am I going to take on this issue? I am not sure I have a stance, all I have are questions and observations. In the following document, I shall attempt to share them with you. What is abortion? Well according to Merriam Webster's dictionary abortion is defined as follows Main Entry abor·tion Pronunciation &-bor-sh&n Function noun Date 1547 1 the termination of a pregnancy after, accompanied by, resulting in, or closely followed by the death of the embryo or fetus as a spontaneous expulsion of a human fetus during the first 1 weeks of gestation -- compare MISCARRIAGE b induced expulsion of a human fetus c expulsion of a fetus by a domestic animal often due to infection at any time before completion of pregnancy -- compare CONTAGIOUS ABORTION MONSTROSITY arrest of development (as of a part or process) resulting in imperfection; also a result of such arrest Roget's thesaurus breaks the word down into two categories for its synonyms. Those two categories are nonpreparation and failure followed by rather extensive lists of words meaning the same. The connotations are obvious. However, I have to ask myself, should we attempt to stop abortion? I have often joked that abortion is fine, after all, stupid people shouldn't breed. Now I find myself thinking about this joke in detail. What is the purpose of life? What, ultimately is hardwired deep in the genes of our species. A need to procreate, a desire to extend our genetic legacy into future generations. Survival of the species. Some animals will kill the young to establish their dominance, allowing only a few to survive. The lucky few. However, a race that kills too many of its young is at genetic dead end. A species that has no concern or regard for its young goes against survival of the species. This may sound like an argument opposing abortion, but consider all the ramifications. On a strictly animal level, an animal that allows its young to die should not breed, least it pass on that trait to future offspring. Consequently, an animal that does so should have its young destroyed, to prevent this trait from passing into the gene pool. I can think of no stronger argument for abortion than this. I remember reading an article once that stated that the fetus would often scream as it was dismembered. Its obvious that this cannot be the case, because it is simply a mass of tissue, a grouping of cells, something that is not yet human, or subhuman is you prefer. In that case dismembering the fetus is no crime. Neither would giving an injection to induce premature labor, inducing a heart attack, or poisoning the fetus so that its heart stops beating. Despite the fact that that the fetus has a human form, eyes, legs, arms, a heart, and often a different blood type than the mother, it isn't human. There is of course plenty of historical precedence to support the fact that something may look human, but is in fact not. Any Plantation owner in the old south of America could tell you as much. Doesn't this sound good? Doesn't it sound familiar? It simply a reflex reaction that causes the screaming-like reaction, not pain, and after all, you can't physically hear any screaming so its obviously isn't there. It's as plain as the nose on your face. After all, the walls were pretty thick at Auschwitz; you couldn't hear any screaming there either. America is obviously falling behind other nations of the world today in industrial capacity. After all, 140's Germany managed to take care of eleven million unwanted pregnancies in just a few short years. America has eliminated three times that many, but has taken five times as long. It is clear to me that we need to step up the process. After all, what better way to cut spending then by eliminating future generations that have the potential to go on welfare and spend my hard earned money. What is this you say? Not every child that is born goes on welfare? Well I can confidently say that 100% of aborted fetuses would go on welfare. After all, if they're never born, how can you prove me wrong? What is this you say? They could be great scientists, leaders, authors, engineers, poets and more. Well again, I can confidently say that you are wrong, because we will never know will we? And of course, abortion is useful in preventing genetic imperfections into our modern gene pool. What a world we could live in if we were free from the horrors of Muscular Dystrophy, Spinal Muscular Atrophy, Tay-Sachs Disease, Neurofibromatoses, diabetes, and the like. Just think about it, you would never have to see people like that again. It wouldn't be necessary to hide them from the world; they quite simply wouldn't be here. Think of it, we would have achieved the master race. Doesn't that sound good? Doesn't that sound familiar? Think about it, just think about it for one moment. Is this the world you want to live in? Is this a world you can accept? I know my answer to that question. Not if I can help it!

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Temperature and Enzyme Activity

If you order your research paper from our custom writing service you will receive a perfectly written assignment on Temperature and Enzyme Activity. What we need from you is to provide us with your detailed paper instructions for our experienced writers to follow all of your specific writing requirements. Specify your order details, state the exact number of pages required and our custom writing professionals will deliver the best quality Temperature and Enzyme Activity paper right on time.

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Introduction

In this lab, our main purpose is to determine the relationship between enzyme and temperature. As we know, enzymes are mainly composed by proteins, which is easily degenerated under high temperature. Also, enzymes are highly specific creatures towards temperature. For example, amylase and catalase are both adapted human body temperature, which is about 7-45¢J. There is an apparent optimum temperature for different enzymes, after which the reaction rate decreases rapidly, because of denaturation of the enzyme. However, the optimum temperature of enzymes depends on the way and which enzyme which the activity is measured. Some enzymes are heat resistant and can work at fairly high temperatures. Our goal in this experiment is to find out the enzyme activity at different temperatures.

Method

In this experiment, we will test the effect of temperature on amylase activity. For that the variable is temperature (5¢J, 7¢J, 50¢J, 80¢J), and the main control variable is pH, which will be standardized my a certain buffer(pH=6.). The reactant will be starch, and tester will be IKI (Iodine potassium iodine). Distilled water is used as control groups. The procedures are as follow

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1. Label test tubes and droppers so that we don¡¦t contaminate the experiment by mixing them together.

. Turn on the temperature maintenance machines, and set 1 at 7¢J, another at 50¢J, the other at 80¢J.

. Set up spot plates to test drops of experiment and control groups, and label each spot with minutes preceding one another.

4. Add 1ml of buffer pH=6. to both control and experimental test tubes.

5. Add 1ml of water to the control group.

6. Add 1ml of starch solution to the experimental test tube.

7. Place experimental test tube (E-7) and control test tube (C-7) in the temperature maintenance machine, which is set to 7¢J.

8. Warm test tubes for 5-10 minutes in order to achieve the desired temperature.

. Get the amylase ready in ice, and the dropper filled with amylase.

10. Place 1~ drops of IKI in the spots indicated 7¢J.

11. Add 0.5ml of amylase to both the control and experimental test tubes, and so the reaction starts. Squeeze and release the dropper gently to mix the solution in the test tubes. Take down the time when amylase is added.

1. Start to take samples at 10 second intervals for the first minute. If there are no significant changes within the first minute, then start to record the change every minute. While you do this, put a drop of the control test tube solution on another spot in correspond to the experimental solution.

1. Record the color changes on the data table.

14. Continue to record the color change in between 1 minute interval, and if need additional time points, wash the spot plates out and re-use them.

15. When the experimental color reaches the same color as the control solution (yellow), all the starch has been hydrolyzed. This is the reaction end point. Take this number down carefully.

Results

During the experiment, we acquired results displayed as below, and also seen in figure1.

5¢J 7¢J 50¢J 80¢J

T=reaction end point 15 10 7 0

1/T=Enzyme Activity 0.06666667 0.1000000000 0.0707070 0

Table1

As seen in this table, we obtained the outcome that 7¢J has the highest enzyme activity (lowest reaction time period), 5¢J comes in second, 50¢J comes third, and there is no end point at 80¢J, meaning during the certain time period, the solution wasn¡¦t able to change color completely. The figure shows a curve with a highest point of 0.1, which occurred at 7¢J, and no enzyme activity at 80¢J.

Conclusion

A predicted graph of amylase reaction with temperature change is shown as below

The graph showed a optimum temperature at 40¢J, and drops quite rapidly afterwards. This suggests that amylase works best at temperature slightly below 40¢J. Therefore body temperature, 7¢J, is certainly ideal for amylase to work under, which is also illustrated in our figure1. In figure1, we obtained an optimum at 7¢J; however, there was still enzyme activity at 50¢J, which differed from the predicted graph. This could be caused by several human errors, such as not turning the temperature maintenance machine high enough, or did not leave the experimental and control test tube in the machine long enough, so the temperature of solution was not 50¢J. Moreover, the enzyme value may be miss measured, that we put more than 0.5ml of enzyme into the test tube, increased the enzyme concentration thus showed enzyme activity at 50¢J.

Another interesting phenomenon we acquired during the experiment is that it took us a lot longer to achieve the end points than the other groups. As seen in the table, it took us 10 minutes for 7¢J experiment and 15minutes for 5¢J experiment while normally it should only take 1 to minutes. We reached the conclusion that either the amylase we used was contaminated by other substances or they denatured during the testing. Maybe we did not put them in the ice correctly. Also, one of our teammates forgot to put amylase into some of the solutions. Even though we re-ran the experiment again, it might affect our results. These are the reasons why our experiment are not as accurate as it was predicted.

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